Endocrinology Research and Practice
Original Article

Investigating the Relationship Between a Low Carbohydrate Diet Score and Inflammatory and Oxidative Stress Biomarkers in Female Students


Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran


Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran


Division of Medical Education, Brighton & Sussex Medical School, Sussex, United Kingdom


Clinical Research Development Unit of Akbar Hospital, Mashhad University of Medical Sciences, Mashhad, Iran


Clinical Research Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Faculty of Medicine, Mashhad, Iran

Endocrinol Res Pract 2023; 27: 121-126
DOI: 10.5152/erp.2023.22153
Read: 440 Downloads: 126 Published: 01 July 2023

Objective: A healthy diet is a major component of lifestyle management for maintaining optimum health. Dietary carbohydrates may induce inflammation and oxidative stress. We aimed to assess the relationship between adherence to a low-carbohydrate diet pattern with oxidative stress status and a panel of blood biomarkers of inflammation in healthy young women.

Methods: In this study, 171 healthy young women participated. We estimated the low-carbohy- drate diet scores using a food frequency questionnaire. The total antioxidant capacity and free radi- cal scavenging activity and malondialdehyde of serum and urine were quantified using the ferric reducing/antioxidant power, α, α-diphenyl-β-picrylhydrazyl, and formation of thiobarbituric acid reactive substances methods. Several routine hematological parameters were measured including white blood cells, neutrophil counts, and mean platelet volume. Neutrophil:lymphocyte ratio and platelet:lymphocyte ratio were derived from these measures.

Results: A higher low-carbohydrate diet score indicates a lower intake of carbohydrates and fiber and a higher intake of protein and fat. A greater adherence to a low-carbohydrate diet pattern was related to a lower level of hematological inflammatory indices including neutrophil count and neutrophil:lymphocyte ratio. Multivariable-adjusted odds ratios (95% CIs) demonstrated that a higher adherence to low-carbohydrate diet (third vs. first tertile) was associated with significantly higher levels of urinary α, α-diphenyl-β-picrylhydrazyl (adjusted odds ratio = 1.10; 95% CI: 1.02-1.20), as well as serum ferric reducing/antioxidant power (adjusted odds ratio=1.05; 95% CI: 1.01-1.011) and α, α-diphenyl-β-picrylhydrazyl (adjusted odds ratio = 1.21; 95% CI: 1.11-1.33).

Conclusions: A greater compliance with a low-carbohydrate-style diet was related to lower levels of inflammatory biomarker and oxidative stress in healthy young women.

Cite this article as: Moradi-binabaj M, Khorasanchi Z, Karbasi S, Ferns GA, Bahrami A. Investigating the relationship between a low carbohydrate diet score and inflammatory and oxidative stress biomarkers in female students. Endocrinol Res Pract. 2023;27(3):121-126.

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