Overexpression of miR-29b in Plasma of Javanese Type 2 Diabetes Mellitus Patients in Semarang, Indonesia

Objective: In diabetes, the miR-29b, also known as redoximiRs, has been shown to regulate oxidative pathways. The aim of this study was to observe the circulation of miR-29b in plasma of Javanese type 2 diabetes mellitus patients at the Primary Health Care Center in Semarang, Indonesia, and its correlation with fasting blood glucose, cholesterol, and triglyceride.

Methods: The expression of miR-29b in type 2 diabetes mellitus groups and control groups was measured by reverse transcriptase polymerase chain reaction (RT-PCR). The 2–ΔΔCq method was used to determine the relative amount of miR-29b. The miR-29b expression was compared with an independent t-test. Furthermore, the correlation between miR-29b expression was analyzed by the Pearson correlation test using SPSS 17.0. Fold change was positive at 14.12 (i.e., upregulation).

Results: There was a significant negative correlation between age and miR-29b expression (P=.000, r=–0.627). Moreover, fasting blood glucose and miR-29b also had a negative correlation (P=.000, r=–0.504).

Conclusion: miR-29b overexpression occurred in plasma of Javanese type 2 diabetes mellitus patients at the Primary Health Care Center in Semarang, Indonesia, i.e., 14.12-fold overexpression. The level of miR-29b expression was negatively correlated with age and fasting blood glucose.

Cite this article as: Tursinawati Y, Kartikadewi A, Yuniastuti A, Susanti R. Overexpression of miR-29b in plasma of javanese type 2 diabetes mellitus patients in Semarang, Indonesia. Turk J Endocrinol Metab. 2022;26(4):208-212.